Single-Molecule Fingerprint Spectrum for Biological Nanopore Sensing

Nanopore technique has been developed as a powerful approach for single-molecule detection. Current nanopore data analysis method mainly involves time-domian process including the amplitude, translocation duration and interval time of current events. However, more abundant information hidden from noise which lacks quantitative to extract them appropriately. In view advantages that there are intrinsic characteristics in during analytes traversing through nanopore, here, we present mutidimensional strategy obtain fingerprint frequency spectrum traces by Hilbert-Huang Transform. The underlying physical chemical was explored behind this when poly(dA)4 aerolysin nanopore. crosslink established between value series characteristic variables included properties analytes, integrity, amino acids residue at mutant site. results have demonstrated existence inside pore, reversible protonation pore lumen number carriers would cause great changes spectrum. pearson correlation coefficient also showed associated with conductance without DNA, while it correlates DNA inside. These indicate non-covalent interaction may be reflected it. This gives multidimensional feature could further utilized application peptide sequencing.